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primary antibodies  (R&D Systems)


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    Structured Review

    R&D Systems primary antibodies
    Primary Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/af2105/Human%2FMouse%2FRat+Vimentin+Antibody/pmc12466173__res-137-1117-s001-39-0-13
    Average 94 stars, based on 70 article reviews
    primary antibodies - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Software:

    Article Title: Placenta-specific protein 1 promotes cell proliferation and invasion in non-small cell lung cancer
    Article Snippet: Cells protein lysates (Millipore) were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to 0.22 μm NC membranes (Sigma, St. Louis, MO, USA), and incubated with specific antibodies. .. Enhanced chemiluminescence (ECL) chromogenic substrate (Beyotime, Shanghai, China) was used to visualize the bands and the intensity of the bands were quantified by densitometry (Quantity One Software; Bio-Rad, Hercules, CA, USA). β-actin antibody (1:5,000) was used as a control, and anti-PLAC1 (1:500) (ab117528), anti-vimentin (1:2,000) (AF2105), anti-AKT (1:5,000) (MAB2055), anti-pAKT (S473) (1:2,000) (AF887) and anti-E-cadherin (1:10,000) (AF648) were purchased from R&D Systems. .. Donkey anti-goat IgG-HRP (1:5,000) (sc-2020) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    Control:

    Article Title: Placenta-specific protein 1 promotes cell proliferation and invasion in non-small cell lung cancer
    Article Snippet: Cells protein lysates (Millipore) were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to 0.22 μm NC membranes (Sigma, St. Louis, MO, USA), and incubated with specific antibodies. .. Enhanced chemiluminescence (ECL) chromogenic substrate (Beyotime, Shanghai, China) was used to visualize the bands and the intensity of the bands were quantified by densitometry (Quantity One Software; Bio-Rad, Hercules, CA, USA). β-actin antibody (1:5,000) was used as a control, and anti-PLAC1 (1:500) (ab117528), anti-vimentin (1:2,000) (AF2105), anti-AKT (1:5,000) (MAB2055), anti-pAKT (S473) (1:2,000) (AF887) and anti-E-cadherin (1:10,000) (AF648) were purchased from R&D Systems. .. Donkey anti-goat IgG-HRP (1:5,000) (sc-2020) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

    other:

    Article Title: Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids
    Article Snippet: : AF2105; RRID: AB_355153 Goat anti-CDH1/E-cadherin R&D Systems Cat. No.: AF648; RRID: AB_355504 Goat anti-FOXA2 R&D Systems Cat.No.

    Article Title: Immunohistochemical Expression Pattern of FGFR1, FGFR2, RIP5, and HIP2 in Developing and Postnatal Kidneys of Dab1 −/− ( yotari ) Mice
    Article Snippet: , Human/Mouse/Rat Vimentin Antibody , AF2105 , Goat , 1:300 , R&DSystems (Minneapolis, MN, USA).

    Immunocytochemistry:

    Article Title: Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids
    Article Snippet: : NBP1-30054; RRID: AB_1968486; 34 publications listed on the manufacturer's website Goat anti-Vimentin R&D Systems Cat.No. .. : AF2105; RRID: AB_355153; 20 publications listed on the manufacturer's website, 6 different data examples (2 ICC, 2 KO validation, 2 IHC) Goat anti-CDH1/E-cadherin R&D Systems Cat. No.: AF648; RRID: AB_355504; 21 publications listed on the manufacturer's website, 7 different data examples (2 WB, 1 FACS, 2 ICC, 1 IHC, 1 RNAscope) Goat anti-FOXA2 R&D Systems Cat.No. .. : AF2400; RRID: AB_2294104; 38 publications listed on the manufacturer's website, 4 different data examples (1 WB, 1 ChiP, 1 ICC, 1 IHC) Rabbit anti-FOXF1 Abcam Cat.No.

    Gene Knockout:

    Article Title: Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids
    Article Snippet: : NBP1-30054; RRID: AB_1968486; 34 publications listed on the manufacturer's website Goat anti-Vimentin R&D Systems Cat.No. .. : AF2105; RRID: AB_355153; 20 publications listed on the manufacturer's website, 6 different data examples (2 ICC, 2 KO validation, 2 IHC) Goat anti-CDH1/E-cadherin R&D Systems Cat. No.: AF648; RRID: AB_355504; 21 publications listed on the manufacturer's website, 7 different data examples (2 WB, 1 FACS, 2 ICC, 1 IHC, 1 RNAscope) Goat anti-FOXA2 R&D Systems Cat.No. .. : AF2400; RRID: AB_2294104; 38 publications listed on the manufacturer's website, 4 different data examples (1 WB, 1 ChiP, 1 ICC, 1 IHC) Rabbit anti-FOXF1 Abcam Cat.No.

    Biomarker Discovery:

    Article Title: Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids
    Article Snippet: : NBP1-30054; RRID: AB_1968486; 34 publications listed on the manufacturer's website Goat anti-Vimentin R&D Systems Cat.No. .. : AF2105; RRID: AB_355153; 20 publications listed on the manufacturer's website, 6 different data examples (2 ICC, 2 KO validation, 2 IHC) Goat anti-CDH1/E-cadherin R&D Systems Cat. No.: AF648; RRID: AB_355504; 21 publications listed on the manufacturer's website, 7 different data examples (2 WB, 1 FACS, 2 ICC, 1 IHC, 1 RNAscope) Goat anti-FOXA2 R&D Systems Cat.No. .. : AF2400; RRID: AB_2294104; 38 publications listed on the manufacturer's website, 4 different data examples (1 WB, 1 ChiP, 1 ICC, 1 IHC) Rabbit anti-FOXF1 Abcam Cat.No.

    Immunohistochemistry:

    Article Title: Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids
    Article Snippet: : NBP1-30054; RRID: AB_1968486; 34 publications listed on the manufacturer's website Goat anti-Vimentin R&D Systems Cat.No. .. : AF2105; RRID: AB_355153; 20 publications listed on the manufacturer's website, 6 different data examples (2 ICC, 2 KO validation, 2 IHC) Goat anti-CDH1/E-cadherin R&D Systems Cat. No.: AF648; RRID: AB_355504; 21 publications listed on the manufacturer's website, 7 different data examples (2 WB, 1 FACS, 2 ICC, 1 IHC, 1 RNAscope) Goat anti-FOXA2 R&D Systems Cat.No. .. : AF2400; RRID: AB_2294104; 38 publications listed on the manufacturer's website, 4 different data examples (1 WB, 1 ChiP, 1 ICC, 1 IHC) Rabbit anti-FOXF1 Abcam Cat.No.

    Western Blot:

    Article Title: Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids
    Article Snippet: : NBP1-30054; RRID: AB_1968486; 34 publications listed on the manufacturer's website Goat anti-Vimentin R&D Systems Cat.No. .. : AF2105; RRID: AB_355153; 20 publications listed on the manufacturer's website, 6 different data examples (2 ICC, 2 KO validation, 2 IHC) Goat anti-CDH1/E-cadherin R&D Systems Cat. No.: AF648; RRID: AB_355504; 21 publications listed on the manufacturer's website, 7 different data examples (2 WB, 1 FACS, 2 ICC, 1 IHC, 1 RNAscope) Goat anti-FOXA2 R&D Systems Cat.No. .. : AF2400; RRID: AB_2294104; 38 publications listed on the manufacturer's website, 4 different data examples (1 WB, 1 ChiP, 1 ICC, 1 IHC) Rabbit anti-FOXF1 Abcam Cat.No.

    FACS:

    Article Title: Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids
    Article Snippet: : NBP1-30054; RRID: AB_1968486; 34 publications listed on the manufacturer's website Goat anti-Vimentin R&D Systems Cat.No. .. : AF2105; RRID: AB_355153; 20 publications listed on the manufacturer's website, 6 different data examples (2 ICC, 2 KO validation, 2 IHC) Goat anti-CDH1/E-cadherin R&D Systems Cat. No.: AF648; RRID: AB_355504; 21 publications listed on the manufacturer's website, 7 different data examples (2 WB, 1 FACS, 2 ICC, 1 IHC, 1 RNAscope) Goat anti-FOXA2 R&D Systems Cat.No. .. : AF2400; RRID: AB_2294104; 38 publications listed on the manufacturer's website, 4 different data examples (1 WB, 1 ChiP, 1 ICC, 1 IHC) Rabbit anti-FOXF1 Abcam Cat.No.

    RNAscope:

    Article Title: Co-development of central and peripheral neurons with trunk mesendoderm in human elongating multi-lineage organized gastruloids
    Article Snippet: : NBP1-30054; RRID: AB_1968486; 34 publications listed on the manufacturer's website Goat anti-Vimentin R&D Systems Cat.No. .. : AF2105; RRID: AB_355153; 20 publications listed on the manufacturer's website, 6 different data examples (2 ICC, 2 KO validation, 2 IHC) Goat anti-CDH1/E-cadherin R&D Systems Cat. No.: AF648; RRID: AB_355504; 21 publications listed on the manufacturer's website, 7 different data examples (2 WB, 1 FACS, 2 ICC, 1 IHC, 1 RNAscope) Goat anti-FOXA2 R&D Systems Cat.No. .. : AF2400; RRID: AB_2294104; 38 publications listed on the manufacturer's website, 4 different data examples (1 WB, 1 ChiP, 1 ICC, 1 IHC) Rabbit anti-FOXF1 Abcam Cat.No.

    Staining:

    Article Title: Mid-old cells are a potential target for anti-aging interventions in the elderly.
    Article Snippet: Counterstaining of each stained slide was performed using Hematoxylin II (790-2208, VentanaMedical Sytems Inc.). .. For IF staining of FFPE tissue sections, the followingprimary antibodieswere incubatedovernight at 4 °C: SAA1, 1:50 (MAB30191, R&D Systems); p21Waf1, 1:100 (ab109520, Abcam); IL1β, 1:100 (ab9722, Abcam); SLIT2, 1:200 (PA5-31133, Invitrogen); p16INK4A, predilution (805-4713, Roche); Vimentin, 1:400 (ab92547, Abcam); Vimentin, 1:100 (AF2105, R&D Systems); Smoothelin, 1:100 (OMA1-06020, Invitrogen); Smoothelin, 1:500 (NBP2-37931, Novus Biologicals); E-cadherin, 1:200 (610181, Becton Dickinson); Ecadherin, 1:100 (ab15148, Abcam); CDX2, 1:100, (235R-15, Cell Margue, Rocklin, CA). ..

    Article Title: Mid-old cells are a potential target for anti-aging interventions in the elderly
    Article Snippet: Counterstaining of each stained slide was performed using Hematoxylin II (790-2208, Ventana Medical Sytems Inc.). .. For IF staining of FFPE tissue sections, the following primary antibodies were incubated overnight at 4 °C: SAA1, 1:50 (MAB30191, R&D Systems); p21 Waf1 , 1:100 (ab109520, Abcam); IL1β, 1:100 (ab9722, Abcam); SLIT2, 1:200 (PA5-31133, Invitrogen); p16 INK4A , predilution (805-4713, Roche); Vimentin, 1:400 (ab92547, Abcam); Vimentin, 1:100 (AF2105, R&D Systems); Smoothelin, 1:100 (OMA1-06020, Invitrogen); Smoothelin, 1:500 (NBP2-37931, Novus Biologicals); E-cadherin, 1:200 (610181, Becton Dickinson); E-cadherin, 1:100 (ab15148, Abcam); CDX2, 1:100, (235R-15, Cell Margue, Rocklin, CA). ..

    Formalin-fixed Paraffin-Embedded:

    Article Title: Mid-old cells are a potential target for anti-aging interventions in the elderly.
    Article Snippet: Counterstaining of each stained slide was performed using Hematoxylin II (790-2208, VentanaMedical Sytems Inc.). .. For IF staining of FFPE tissue sections, the followingprimary antibodieswere incubatedovernight at 4 °C: SAA1, 1:50 (MAB30191, R&D Systems); p21Waf1, 1:100 (ab109520, Abcam); IL1β, 1:100 (ab9722, Abcam); SLIT2, 1:200 (PA5-31133, Invitrogen); p16INK4A, predilution (805-4713, Roche); Vimentin, 1:400 (ab92547, Abcam); Vimentin, 1:100 (AF2105, R&D Systems); Smoothelin, 1:100 (OMA1-06020, Invitrogen); Smoothelin, 1:500 (NBP2-37931, Novus Biologicals); E-cadherin, 1:200 (610181, Becton Dickinson); Ecadherin, 1:100 (ab15148, Abcam); CDX2, 1:100, (235R-15, Cell Margue, Rocklin, CA). ..

    Article Title: Mid-old cells are a potential target for anti-aging interventions in the elderly
    Article Snippet: Counterstaining of each stained slide was performed using Hematoxylin II (790-2208, Ventana Medical Sytems Inc.). .. For IF staining of FFPE tissue sections, the following primary antibodies were incubated overnight at 4 °C: SAA1, 1:50 (MAB30191, R&D Systems); p21 Waf1 , 1:100 (ab109520, Abcam); IL1β, 1:100 (ab9722, Abcam); SLIT2, 1:200 (PA5-31133, Invitrogen); p16 INK4A , predilution (805-4713, Roche); Vimentin, 1:400 (ab92547, Abcam); Vimentin, 1:100 (AF2105, R&D Systems); Smoothelin, 1:100 (OMA1-06020, Invitrogen); Smoothelin, 1:500 (NBP2-37931, Novus Biologicals); E-cadherin, 1:200 (610181, Becton Dickinson); E-cadherin, 1:100 (ab15148, Abcam); CDX2, 1:100, (235R-15, Cell Margue, Rocklin, CA). ..

    Incubation:

    Article Title: Mid-old cells are a potential target for anti-aging interventions in the elderly
    Article Snippet: Counterstaining of each stained slide was performed using Hematoxylin II (790-2208, Ventana Medical Sytems Inc.). .. For IF staining of FFPE tissue sections, the following primary antibodies were incubated overnight at 4 °C: SAA1, 1:50 (MAB30191, R&D Systems); p21 Waf1 , 1:100 (ab109520, Abcam); IL1β, 1:100 (ab9722, Abcam); SLIT2, 1:200 (PA5-31133, Invitrogen); p16 INK4A , predilution (805-4713, Roche); Vimentin, 1:400 (ab92547, Abcam); Vimentin, 1:100 (AF2105, R&D Systems); Smoothelin, 1:100 (OMA1-06020, Invitrogen); Smoothelin, 1:500 (NBP2-37931, Novus Biologicals); E-cadherin, 1:200 (610181, Becton Dickinson); E-cadherin, 1:100 (ab15148, Abcam); CDX2, 1:100, (235R-15, Cell Margue, Rocklin, CA). ..



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